Fig. 2From: CircZDHHC20 represses the proliferation, migration and invasion in trophoblast cells by miR-144/GRHL2 axisCircZDHHC20 acted as a molecular sponge of miR-144. a Schematic of circZDHHC20 illustrating position of the miR-144-binding site and mutated the miR-144-binding site. b, c Relative luciferase activity in HTR-8/SVneo cells cotransfected with circZDHHC20-WT or circZDHHC20-MUT and miR-con mimic, miR-144 mimic, anti-miR-con or anti-miR-144. d The enrichment of circZDHHC20 and miR-144 in the RISC of HTR-8/SVneo cells using anti-Ago2 or IgG antibody, with Input content as positive control. e The expression of miR-144 by qRT-PCR in HTR-8/SVneo cells transfected with negative control plasmid (vector), circZDHHC20 overexpression plasmid (circZDHHC20), si-con or si-circZDHHC20. *P < 0.05Back to article page