Fig. 3

MiR-144 was involved in the regulation of circZDHHC20 on trophoblast cell proliferation, migration, and invasion. MTS assay for cell proliferation (a, b), wound healing assay for cell migration (c, d), transwell assay for cell invasion (e, f) in HTR-8/SVneo cells transfected with si-con, si-circZDHHC20, si-circZDHHC20 + anti-miR-con, si-circZDHHC20 + anti-miR-144, negative control plasmid (vector), circZDHHC20 overexpression plasmid (circZDHHC20), circZDHHC20 + miR-con mimic, or circZDHHC20 + miR-144 mimic. *P < 0.05