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Fig. 4 | Cancer Cell International

Fig. 4

From: CircZDHHC20 represses the proliferation, migration and invasion in trophoblast cells by miR-144/GRHL2 axis

Fig. 4

GRHL2 was a direct target of miR-144. a Schematic of the complementary site for miR-144 in the 3′-UTR of GRHL2 and the mutation of the seed region. b, c Relative luciferase activity in HTR-8/SVneo cells introduced with GRHL2-WT or GRHL2-MUT together with miR-con mimic, miR-144 mimic, anti-miR-con or anti-miR-144. d The enrichment of GRHL2 and miR-144 in the RISC of HTR-8/SVneo cells using anti-Ago2 or IgG antibody, with Input content as positive control. e qRT-PCR for GRHL2 mRNA in placental tissues from 26 PE patients and 15 healthy volunteers. f Western blot for GRHL2 protein level in HTR-8/SVneo cells transfected with miR-con mimic, miR-144 mimic, anti-miR-con, or anti-miR-144. *P < 0.05

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