Fig. 5

GRHL2 mediated the regulatory effects of miR-144 on trophoblast cell proliferation, migration and invasion. Cell proliferation by MTS assay (a, b), cell migration by wound healing assay (c, d), cell invasion by transwell assay (e, f) in HTR-8/SVneo cells transfected with miR-con mimic, miR-144 mimic, miR-144 mimic + negative control plasmid (vector), miR-144 mimic + GRHL2 overexpression plasmid, anti-miR-con, anti-miR-144, anti-miR-144 + si-con, or anti-miR-144 + si-GRHL2. *P < 0.05