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Fig. 3 | Cancer Cell International

Fig. 3

From: ZEB1-AS1/miR-133a-3p/LPAR3/EGFR axis promotes the progression of thyroid cancer by regulating PI3K/AKT/mTOR pathway

Fig. 3

MiR-133a-3p directly targets LPAR3 and EGFR. a Venn diagram illustrated the potential miRNAs for LPAR3 and EGFR. b RNA pull down assay revealed the possible miRNAs for LPAR3 and EGFR. c MiR-133a-3p expression in TC tissues and matched normal tissues was detected via qRT-PCR. d MiR-133a-3p expression in TC cell lines and normal cell line was examined via qRT-PCR. e The association between miR-133a-3p and LPAR3 or EGFR. f MiR-133a-3p was presented to have binding sites on LPAR3 or EGFR. g qRT‐PCR was applied to test miR-133a-3p expression in cells transfected with miR-133a-3p mimics or NC mimics. h The interaction between miR-133a-3p and LPAR3 or EGFR was confirmed by luciferase reporter assay. i LPAR3 or EGFR expression in transfected cells was detected by qRT-PCR. j CCK-8 assay was conducted to detect the effect of indicated vectors on cell proliferation. k Flow cytometry analysis was utilized to assess the effect of indicated vectors on cell apoptosis. *P < 0.05, **P < 0.01, ***P < 0.001

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