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Fig. 2 | Cancer Cell International

Fig. 2

From: SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis

Fig. 2

SP1 induces LINC00958 overexpression by serving as a transcription activator. a QRT-PCR analysis was performed to investigate the expression of SP1 after transfecting with pcDNA3.1 and pcDNA3.1/SP1. b QRT-PCR was performed to study the effect of pcDNA3.1/SP1 on LINC00958 expression. c The putative SP1 binding motif in − 2000 bp of human LINC00958 promoter. d LINC00958 promoter was divided into four sectional fragments. e ChIP assay using antibody targeting SP1 and IgG was performed to detect the affinity of SP1 with LINC00958 promoter. f The full LINC00958 promoter (P FL) and LINC00958 P1 deleted (P D) was constructed. g ChIP assay using antibody targeting SP1 and IgG was performed to determine the relative enrichment of LINC00958 promoter. h pGL3-P1-WT, pGL3-P1-MUT1 and pGL3-P1-MUT2 luciferase vectors were constructed. i HEK-293T cells were transfected with pcDNA3.1 and pcDNA3.1/SP1 and luciferase reporter assays was performed among P1-WT, P1-MUT1 and P1-MUT2. **P < 0.01

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