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Fig. 3 | Cancer Cell International

Fig. 3

From: SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis

Fig. 3

LINC00958 directly interacts with miR-625-5p and miR-625-5p inhibition counteracts the effects of silencing LINC00958 on LAD progression. a, b Subcellular fractionation and FISH assays were used to determine the situation of LINC00958. c QRT-PCR analysis was performed to determine candidate miRNAs expression after silencing LINC00958. d QRT-PCR assay was performed to detect the expression of miR-625-5p in LAD cell lines and normal pulmonic cell line. e Potential miR-625-5p binding site with LINC00958 detected by starBase. f Luciferase reporter assay found that miR-625-5p mimics could attenuate the luciferase activity of LINC00958-WT. g RNA pull down showed enriched miR-625-5p by biotin-labeled LINC00958-WT. h The inhibitory efficiency of miR-625-5p inhibitor was examined by qRT-PCR. im A list of functional experiments was conducted in PC9 and A549 cell lines in a rescue manner. **P < 0.01

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