Fig. 6

Microscopy and Flow cytometry analysis of A375 melanoma cancer cells after pre-irradiation and treatment with GA for 24 h. Panel I: invert microscopy images (×40), panel II: AO/EB double staining, panel III: the apoptotic rates (annexin V-FITC/PI dual staining) and panel IV: colony-forming ability. a 0 µg/ml (control), and b 25 µg/ml of GA at dark condition, c 0 µg/ml and d 25 µg/ml of GA under low-laser irradiation. e Histogram showing percentages of apoptotic and necrotic cells in the dark and irradiation groups treated with irradiation and GA. Percent of apoptotic and necrotic cells obtained from (panel III)