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Fig. 4 | Cancer Cell International

Fig. 4

From: EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway

Fig. 4

The effect of EphA5 knockdown on EMT was mediated by activating Wnt/β‑catenin signaling pathway. a, b Western blot was used to determine the expression of the key factors in Wnt/β‑catenin signaling (total and phosphorylated GSK-3β, β-catenin), and the downstream targets of Wnt/β‑catenin signaling(c-Myc and CyclinD1) in KYS150 and KYSE450 cells. c, d Immunofluorescence analysis showed β-catenin and phosphorylated GSK-3β localization and expression. Nuclei were counterstained with DAPI (blue). *P < 0.05, **P < 0.01, ***P < 0.001 versus NC group

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