Fig. 1

SMAD6 knockdown inhibits RB cell proliferation, migration and epithelial-mesenchymal transition (EMT). a RT-qPCR measured the relative expression of SMAD6 in RB and normal tissue samples. b RT-qPCR detected the relative expression of SMAD6 in four RB cells (WERI-Rb1, Y79, HXO-RB44, SO-Rb50) and one normal ARPE-19 cell line. c Western blot analyzed the protein levels of SMAD6 in four RB cell lines and the noncancerous ARPE-19 cell line. d, e sh-SMAD6-1, sh-SMAD6-2 or sh-SMAD6-3 were introduced into RB cells. RT-qPCR and western blot confirmed the knockdown efficiency of SMAD6. f CCK-8 assay was conducted for detection of cell proliferation. g Transwell assay was carried out for measurement of cell migration. h Western blot examined the levels of E-cadherin, N-cadherin and Vimentin. Experiments were conducted thrice. *p < 0.05, **p < 0.01