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Fig. 2 | Cancer Cell International

Fig. 2

From: SMAD6, positively regulated by the DNM3OS-miR-134-5p axis, confers promoting effects to cell proliferation, migration and EMT process in retinoblastoma

Fig. 2

SMAD6 expression is negatively regulated by microRNA-134-5p. a RT-qPCR detected miR-134-5p expression in four RB cell lines by comparing to the normal cell line (ARPE-9). b RT-qPCR examined miR-134-5p expression in RB and normal tissue samples. c Pearson correlation test about the expression correlation between miR-134-5p and SMAD6. d Predicted binding site between SMAD6 and miR-134-5p obtained from the starBase v3.0 website. e Relative luciferase activity of SMAD6-WT and SMAD6-Mut was measured in indicated cells with luciferase reporter assay. f RT-qPCR measured SMAD6 mRNA levels after upregulation of miR-134-5p in indicated RB cells. g Western blot tested SMAD6 protein levels after transfection of miR-134-5p mimics. Experiments were conducted thrice. *p < 0.05, **p < 0.01

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