Fig. 3
From: ALKBH5-mediated m6A demethylation of lncRNA PVT1 plays an oncogenic role in osteosarcoma
ALKBH5 demethylates PVT1 transcripts. a MeRIP assay was performed to detect the effect of ALKBH5 knockdown on m6A modification of PVT1 in MG63 and H2OS cells. b MeRIP assay was performed to detect the effect of ALKBH5 overexpression on m6A modification of PVT1 in 143B cells. c Luciferase reporter containing wild-type or m6A consensus sequence mutant PVT1 was transfected into MG63 and U2OS cells with ALKBH5 knockdown. The luciferase activity was then detected. d Luciferase reporter containing wild-type or m6A consensus sequence mutant PVT1 was transfected into 143B cells with ALKBH5 overexpression. The luciferase activity was then detected. *p < 0.05