Fig. 4
From: ALKBH5-mediated m6A demethylation of lncRNA PVT1 plays an oncogenic role in osteosarcoma
YTHDF2 is crucial for ALKBH5-mediated suppression of PVT1 degradation. a The RIP assays using YTHDF2, YTHDF3 and YTHDC2 antibodies were carried out in MG63 and H2OS cells. b The effect of ALKBH5 knockdown on the interaction between YTHDF2 and PVT1 was measured using anti-YTHDF2 RIP assay in MG63 and H2OS cells. c The effect of ALKBH5 overexpression on the interaction between YTHDF2 and PVT1 was measured using anti-YTHDF2 RIP assay in 143B cells. d The YTHDF2 expression was knocked down in ALKBH5-overexpressed 143B cells. e The PVT1 level in ALKBH5-overexpressed 143B cells transfected with YTHDF2 shRNA. f The stability of PVT1 over time in ALKBH5-overexpressed 143B cells transfected with YTHDF2 shRNA was measured by qRT-PCR relative to time 0. *p < 0.05, ***p < 0.001