Fig. 6

ALKBH5 promotes OS growth partially through PVT1. a PVT1 was overexpressed in MG63 and H2OS cells with ALKBH5 knockdown. b Restoration of PVT1 expression partly rescued the suppression of MG63 and H2OS cell proliferation mediated by ALKBH5 knockdown as evidenced by CCK-8 assay. c Restoration of PVT1 expression partly rescued the suppression of MG63 and H2OS cell proliferation mediated by ALKBH5 knockdown as evidenced by colony formation assay. d PVT1 was silenced in 143B cells with ALKBH5 overexpression. e PVT1 knockdown partly attenuated the promotion of 143B cell proliferation mediated by ALKBH5 overexpression as evidenced by CCK-8 assay. f PVT1 knockdown partly attenuated the promotion of 143B cell proliferation mediated by ALKBH5 overexpression as evidenced by colony formation assay. g Indicated stable 143B cells were subcutaneously injected into nude mice. The tumor growth curve was measured. h The tumor weight from (g) was measured. *p < 0.05, **p < 0.01