Fig. 2

Liver cancer cells suffer both necrosis and apoptosis following P22077 treatment. a, b The doses of P22077 was indicated and cell viability was measured with CCK-8 assays in LO2, SMMC-7721, SK-Hep1, HepG2 and Huh7 cells for 24 h or 48 h. b data were presented as mean ± SD. c Three HCC cell lines including SMMC-7721, Huh7 and SK-Hep1 were treated with P22077 for 24 h and stained with PI and Annexin V. Cells were analyzed using a flow cytometry. d Treatment of HCC cells with 10 µM or 20 µM P22077 for 24 h resulted in a significant death of cells as determined by phase contrast microscopy. All *P < 0.05 was considered significant