Fig. 3
From: Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis
RHPN1-AS1 directly interacts with miR-7-5p and inhibits miR-7-5p expression. a, b Subcellular fractionation and FISH assays were performed to detect the subcellular location of RHPN1-AS1. c qRT-PCR analysis was performed to evaluate miRNA expression after silencing RHPN1-AS1. d qRT-PCR analysis was conducted to investigate the expression status of miR-7-5p in CRC cell lines and normal cell lines. e Putative and mutated miR-7-5p binding site in the sequence of RHPN1-AS1 was shown by utilizing Starbase. f Dual luciferase reporter assays were performed to study the interaction between miR-7-5p and RHPN1-AS1. g RNA pull down was performed to investigate the association between miR-7-5p and RHPN1-AS1. h The inhibition efficiency of miR-7-5p inhibitor was examined by qRT-PCR. i EdU was performed to detect cell proliferation in sh-Ctrl, sh-RHPN1-AS1#1 or sh-RHPN1-AS1#1 + inhibitor transfected groups. j Flow cytometry was conducted to investigate apoptosis of cells transfected by sh-Ctrl, sh-RHPN1-AS1#1 or sh-RHPN1-AS1#1 + inhibitor. k, l Transwell assays were used to evaluate migration and invasion of cells transfected by sh-Ctrl, sh-RHPN1-AS1#1 or sh-RHPN1-AS1#1 + inhibitor. ns meant no significance. **P < 0.01