Fig. 4

MiR-7-5p targets OGT in CRC. a qRT-PCR was performed to study target gene expression change after overexpressing miR-7-5p. b qRT-PCR analysis was conducted to detect OGT expression in CRC cell lines and normal healthy cell lines. c the binding site between miR-7-5p and OGT was predicted by Starbase, and the corresponding mutated binding site was shown. d Luciferase reporter was performed to detect the association between OGT and miR-7-5p in HEK-293T. e RIP assay was conducted to determine the physical interaction among miR-7-5p and RHPN1-AS1 and OGT. f The inhibiting efficiency of sh-OGT plasmid was investigated by qRT-PCR. g EdU was performed to evaluate cell proliferation under sh-Ctrl or sh-OGT#1 transfection condition. h Flow cytometry was conducted to examine cell apoptosis when knocking down OGT and sh-Ctrl was control group. i, j. Transwell assays were used to detect cell migration and invasion in response to silencing OGT. k Western blot was performed to measure the expression of EMT-related proteins in sh-Ctrl or sh-OGT#1 transfected cells. ns meant no significance. ^**P < 0.01