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Fig. 5 | Cancer Cell International

Fig. 5

From: Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Fig. 5

OGT overexpression rescues the oncogenic function of RHPN1-AS1. a qRT-PCR analysis was used to determine the expression of OGT after pcDNA3.1 and pcDNA3.1/OGT plasmid transfection. b, c qRT-PCR and western blot were performed to determine the OGT mRNA and protein expression. d CCK-8 assay was performed to evaluate cell vitality. e TUNEL assay was conducted to examine cell apoptosis capacity. f, g Transwell assays were performed to investigate cell migration and invasion abilities respectively. h Western blot was performed to measure EMT-related proteins expression change. i The photos of tumor in different transfection groups were taken. Xeograft tumor growth curve was plotted after four-week inoculation of HCT-116 cells transfected with sh-RHPN1-AS1#1. J, k Xeograft tumor volume and weight were measured in sh-Ctrl and sh-RHPN1-AS1 transfected cells in vivo. **P < 0.01

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