Fig. 4
From: Capicua suppresses colorectal cancer progression via repression of ETV4 expression
CIC deficiency-mediated promotion of CRC progression is due to derepression of ETV4. a qRT-PCR analysis of PEA3 group gene expression levels in control and CIC-deficient (CIC KO) CRC cells (HCT116 and HT29). b Western blot analysis presenting upregulation of ETV4 expression in CIC KO CRC cells. c qRT-PCR analysis for ETV4 mRNA levels in control and CIC KO CRC cells transfected with either control (siNC) or ETV4 siRNA (siETV4). d–f Cell growth assay (d), Matrigel invasion assay (e), and trans-well migration assay (f) of control and CIC knockout CRC cells treated with either siNC or siETV4. Three independent experiments were performed. All error bars indicate s.e.m. *P < 0.05, **P < 0.01, and ***P < 0.001