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Fig. 2 | Cancer Cell International

Fig. 2

From: Demonstration of anti-tumour bystander killing with prodrug-preloaded suicide gene-engineered tumour cells: a potential improvement for cancer therapeutics

Fig. 2

Versenised-GCV pre-treated PA-STK cells but not trypsinised cells, induce bystander killing. PA-STK cells were cultured with 50 μM GCV for 16 h and then either harvested by trypsin treatment or by versene treatment with no use of trypsin. They were then mixed with either trypsinised or versenised PA-1 cells in suspension and then plated for analysis of bystander killing by the MTT assay 5 days later. Control refers to the PA-STK and PA-1 cells mixed but not exposed to GCV. N.B.E, is the normal bystander effect resulting from co-culture of PA-STK and PA-1 cells in the continuos presence of GCV. TT refers to trypsinised PA-STK mixed with trypsinised PA-1 cells. T V refers to trypsinised PA-STK mixed with versenised PA-1. VT indicates versenised PA-STK mixed with trypsenised PA-1. VV indicates versenised PA-STK mixed with versenised PA-1. The total number of cells was 5 × 105/well of 96-well tissue culture plate. In all mixing experiments the ratios of PA-STK to PA-1 cells was 50% and the GCV concentration was 50 μM. Error bars represent standard error of the mean. Representative of three similar experiments

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