Fig. 6
Pre-treated human mesothelioma CRL-5830-STK induce bystander killing of a CRL-5830 cells and b CRL-5820 cells. The indicated ratios of CRL-5830-STK cells were mixed and plated at an initial cell density of 5 × 104/well in 96-well plates. In one set the CRL-5830-STK cells were GCV pre-loaded (pre-treated) by incubation in the presence of 30 μM of GCV for 16 h. In the other set (treated) there was no GCV pre-loading, but 30 μM GCV was added to the culture for the duration of the experiment. These cultures were incubated for 5 days and  % cell survival was assessed by MTT assay. Standard error of the mean is shown