Fig. 2

Overexpression of hsa_circ_0003159 inhibits proliferation, migration and invasion but induces apoptosis in GC cells. a, b The expression of hsa_circ_0003159 was detected in NUGC-3 and AGS cells transfected with hsa_circ_0003159 or vector by qRT-PCR. c, d The proliferation of NUGC-3 and AGS cells transfected with hsa_circ_0003159 or vector was measured at 24, 48 and 72 h by CCK-8 assay. e The apoptotic rate of NUGC-3 and AGS cells transfected with hsa_circ_0003159 or vector was detected at 72 h by flow cytometry. f The migrated and invasive abilities of NUGC-3 and AGS cells transfected with hsa_circ_0003159 or vector were analyzed at 24 h by transwell assay. Scale bar: 50 μm. g The protein levels of CyclinD1, Cleaved-caspase-3 and MMP-9 were measured in NUGC-3 and AGS cells transfected with hsa_circ_0003159 or vector at 72 h by western blot. *P < 0.05