Fig. 3

Hsa_circ_0003159 is a sponge of miR-223-3p in GC cells. a The binding sites of hsa_circ_0003159 and miR-223-3p were predicted by Circular RNA Interactome. b, c Luciferase activity was measured in NUGC-3 and AGS cells co-transfected with WT hsa_circ_0003159 or MUT hsa_circ_0003159 and miR-NC or miR-223-3p. d The expression of miR-223-3p was detected in GC tissues and normal samples (n = 55) by qRT-PCR. e The linear association between the levels of hsa_circ_0003159 and miR-223-3p in GC tissues was analyzed. f The level of miR-223-3p was examined in GC cell lines (NUGC-3 and AGS) and GES-1 cells by qRT-PCR. g The abundance of hsa_circ_0003159 was measured in NUGC-3 and AGS cells transfected with si-NC or si-hsa_circ_0003159 by qRT-PCR. h, i The expression of miR-223-3p was detected in NUGC-3 and AGS cells transfected with vector, hsa_circ_0003159, si-NC or si-hsa_circ_0003159 by qRT-PCR. *P < 0.05