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Fig. 5 | Cancer Cell International

Fig. 5

From: Hsa_circ_0003159 inhibits gastric cancer progression by regulating miR-223-3p/NDRG1 axis

Fig. 5

NDRG1 is regulated by hsa_circ_0003159 and miR-223-3p in GC cells. a The binding sites of miR-223-3p and NDRG1 were predicted by starBase. b, c Luciferase activity was measured in NUGC-3 and AGS cells co-transfected with NDRG1 3′UTR-WT or NDRG1 3′UTR-MUT and miR-NC or miR-223-3p. d, e The expression levels of NDRG1 mRNA and protein were detected in GC tissues and normal tissues by qRT-PCR and western blot. f, g The association between expression of NDRG1 mRNA and miR-223-3p or hsa_circ_0003159 in GC tissues was assessed. h, i The mRNA and protein levels of NDRG1 were measured in GC cells (NUGC-3 and AGS) and GES-1 cells by qRT-PCR and western blot. j, k The mRNA and protein levels of NDRG1 were measured in NUGC-3 and AGS cells transfected with anti-miR-223-3p or anti-NC by qRT-PCR and western blot. l, m The mRNA and protein levels of NDRG1 were examined in NUGC-3 and AGS cells transfected with vector, hsa_circ_0003159, hsa_circ_0003159 and miR-NC or miR-223-3p by qRT-PCR and western blot. *P < 0.05

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