Fig. 6

Knockdown of NDRG1 attenuates the effect of hsa_circ_0003159 on proliferation, migration and apoptosis in GC cells. a, b The mRNA and protein levels of NDRG1 were measured in NUGC-3 and AGS cells transfected with si-NC or si-NDRG1 by qRT-PCR and western blot. Cell proliferation (c, d), apoptosis (e), migration (f), invasion (g) and protein levels of CyclinD1, Cleaved-caspase-3 and MMP-9 (h, i) were examined in NUGC-3 and AGS cells transfected with vector, hsa_circ_0003159, hsa_circ_0003159 and si-NC or si-NDRG1 by CCK-8, flow cytometry, transwell and western blot assays, respectively. *P < 0.05