Fig. 1

CircPIP5K1A is markedly elevated and characterized with loop structure in GC. a The expression of circPIP5K1A in 49 pairs GC tissues and contiguous normal tissues was detected by RT-qPCR. b Overall survival curve of GC patients with high or low circPIP5K1A expression, determined by Kaplan–Meier analysis and log-rank test. c The expression of circPIP5K1A in GC cell lines (BGC-823, MGC-803, HGC-27 and MKN-45) and normal GES-1 cells was examined by RT-qPCR. d The genomic location and splicing pattern of circPIP5K1A was displayed. e Nucleic acid electrophoresis delineated that divergent primers amplified circPIP5K1A from cDNA, but not from gDNA. GAPDH was used as a negative control. f The resistance of circPIP5K1A and PIP5K1A mRNA to ActD was detected by RT-qPCR assay in MKN-45 and HGC-27 cells. g RT-qPCR assay was carried out to determine the abundance of circPIP5K1A and linear PIP5K1A mRNA in MKN-45 and HGC-27 cells treated with RNase R (normalized to mock treatment). ^**P < 0.01