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Fig. 3 | Cancer Cell International

Fig. 3

From: CircPIP5K1A facilitates gastric cancer progression via miR-376c-3p/ZNF146 axis

Fig. 3

CircPIP5K1A sponges miR-376c-3p in GC. a Subcellular fractionation assay was conducted to detect the distribution of circPIP5K1A in MKN-45 and HGC-27 cells. b Five miRNAs (miR-616-3p, miR-2681-5p, miR-376c-3p, miR-642b-3p and miR-642a-3p) was obtained from starBase. c The binding ability between circPIP5K1A and the above mentioned miRNAs was confirmed by RNA pull-down assay. d On the basis of starBase, there existed a binding site between circPIP5K1A and miR-376c-3p. e Luciferase reporter assay validated the interaction between circPIP5K1A and miR-376c-3p. f The expression of miR-376c-3p in GC cell lines and normal GES-1 cells was examined by RT-qPCR. g The expression of miR-376c-3p was detected by RT-qPCR in MKN-45 and HGC-27 cells transfected with sh-circPIP5K1A#1 or sh-NC. h The inhibitory efficiency of miR-376c-3p was evaluated by RT-qPCR assay in MKN-45 and HGC-27 cells. i–j CCK-8, colony formation as well as EdU analyses were utilized to examined the proliferation ability of transfected cells. k The capability of cell migration and invasion was evaluated by transwell. l The apoptosis ability of transfected cells was analyzed by TUNEL and flow cytometry assays. *P < 0.05, **P < 0.01

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