Fig. 3

MiR-135a-5p was a direct target of DANCR. a The potential binding sites of DANCR and miR-135a-5p were predicted by starBase v2.0. b, c The luciferase activity was measured in LN229 and U251 cells co-transfected with DANCR-WT or DANCR-MUT and miR-135a-5p or miR-NC. d RIP assay was performed to detect DANCR enrichment level in IgG or Ago2 immunoprecipitation complex of LN229 and U251 cells. e The level of miR-135a-5p was examined in glioma tissues (n = 33) and normal tissues (n = 33) by qRT-PCR. Statistical analyses were conducted by Wilcoxon signed–rank test. f The abundance of miR-135a-5p was analyzed in glioma cells and NHAs cells by qRT-PCR. g The expression of miR-135a-5p was analyzed in LN229 and U251 cells transfected with DANCR, pcDNA, or DANCR-MUT. **P < 0.01, ***P < 0.001, ****P < 0.0001