Fig. 5

DANCR regulated BMI1 expression by sponging miR-135a-5p in glioma cells. a The putative binding sites of BMI1 and miR-135a-5p were predicted by Tarbase v8.0. b, c Luciferase activity was measured in LN229 and U251 cells co-transfected with BMI1 3′UTR-MUT or BMI1 3′UTR-WT and miR-135a-5p or miR-NC. d The enrichment of BMI1was detected in LN229 and U251 cells transfected with miR-135a-5p or miR-NC after RIP assay. e The abundance of BMI1was measured in glioma tissues and normal tissues by qRT-PCR. The statistical significance of differences was determined using the Wilcoxon signed-rank test. f The abundance of BMI1 protein was analyzed in glioma cells and NHAs cells by western blot. g, h The protein of BMI1 expression was measured in LN229 and U251 transfected with si-DANCR or si-NC by western blot. i The association between BMI1 mRNA level and miR-135a-5p abundance was analyzed in glioma tissues. j, k The correlation between DANCR mRNA level and miR-135a-5p or BMI1 expression was measured in glioma tissues. **P < 0.01, ***P < 0.001, ****P < 0.0001