Fig. 4
Silencing of Siah1 promotes proliferation the of CRC cells. a RNAi-silencing of Siah1 in specific shRNA-transduced stable SW480 and HCT116 cells by Western blot. α-Tubulin was used as a loading control. b, c Knockdown of endogenous Siah1 promoted cell growth, as assessed by MTT assays (b) and colony formation assays (c). d Silencing of Siah1 promotes the growth ability of SW480 and HCT116, as determined by Soft agar assays. Colonies containing > 50 cells were scored. Each error bar represents the mean ± SD from3 independent experiments. Scale bar: 50 μm. e, f SW480/GV248 and SW480/Siah1-shRNA cells (2 × 106) were injected in the hindlimbs of nude mice (n = 6). The volumes of tumor were measured on the indicated days. Panel upper shows tumors after inoculation. Data points are displayed as the mean tumor volumes ± SD (lower panel). f The tumor histological sections were viewed H&E staining and IHC staining using an antibody against Siah1 and Ki-67 (left), right panel shows average percentage of staining cells among the total cell as the Ki-67 index. Scale bar: 50 μm