Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: Downregulation of Siah1 promotes colorectal cancer cell proliferation and migration by regulating AKT and YAP ubiquitylation and proteasome degradation

Fig. 6

Siah1 regulates the activity of MAPK, PI3K-AKT and Hippo pathways by promoting the ubiquitylation of AKT and YAP in CRC cells. a The bioinformatics analysis revealed that the MAPK, Hippo, Wnt, and VEGF signaling pathways were significantly enriched. b Western blot analysis of the expression of the indicated proteins in CRC cells with Siah1 stable overexpression. c Western blot analysis of the expression of the indicated proteins in the CRC cells with Siah1 knockdown. d Ascertain the interaction between Siah1 and AKT/YAP through Co-IP. e Ubiquitylation detection assays analysis of the K48-linked polyubiquitin levels of AKT and YAP in CRC cells treated with MG132

Back to article page