Fig. 1

Evi5 is required for LSCC cells proliferation both in vitro and in vivo. a Western blot analysis of the whole cell lysates (WCLs) derived from TU212 cells infected with the indicated shRNA lentiviruses. b The cell counting assay for TU212 cells infected with the indicated shRNA lentiviruses. c Clonogenic assay of TU212 cells infected with the indicated shRNA lentiviruses. d Evi5 KO TU212 cells were generated by CRISPR assay and detected by western blot. e The cell counting assay for control or Evi5 KO TU212 cells. f Clonogenic assay of control or Evi5 KO TU212 cells. g Cell cycle analyses of control or Evi5 KO TU212 cells. Percentages of cells in each cell cycle stage are reported as the averages of triplicate experiments. h Each nude mouse was subcutaneously injected with 1 × 107 control or Evi5 KO TU212 cells for three weeks. Tumour growth was measured using a caliper at the indicated times after injection. n = 5 for nude mice injected with control or Evi5 KO TU212 cells, separately. ***p < 0.001. The image shows a representative mouse injected with the indicated cells for each group. i Tumor weights were measured after mice were sacrificed. **p < 0.05