Fig. 2
From: The Evi5 oncogene promotes laryngeal cancer cells proliferation by stabilizing c-Myc protein
Evi5 regulated the stability of c-Myc. a Control or Evi5 KO TU212 cells were harvested and analyzed by western for the indicated proteins. b The mRNA levels of p21, cyclin D1 and c-Myc genes were analyzed by Q-PCR. c Evi5 KO TU212 cells were treated with 10 μM MG132 and analyzed by western for the indicated proteins. d TU212 cells were transfected with control or Flag-Evi5 for 36 h, cells were harvested and analyzed by western for the indicated proteins. e The mRNA levels of c-Myc in (D) analyzed by Q-PCR. f The cell lysates of indicated laryngeal cells were subjected to immunoblot analysis with anti-Evi5 and anti-c-Myc antibodies. g 293T cells were co-transfected EV or Flag-Evi5 with a c-Myc-dependent luciferase reporter plasmid (p4 × E-SVP-Luc) and pRL-TK as an internal control, for 48 h. The cells were then assayed for relative luciferase activity using the Dual Luciferase Reporter Assay System. Results are expressed relative to the activity in vector control. Data are mean ± SD of triplicates from a representative experiment