Fig. 4
From: The Evi5 oncogene promotes laryngeal cancer cells proliferation by stabilizing c-Myc protein
Evi5 antagonizes FBXW7-mediated c-Myc ubiquitination and degradation. a Evi5 KO TU212 cells were treated with 1 μM MLN4924 and analyzed by western for the indicated proteins. b Evi5 KO TU212 cells were transfected with Flag-control or Flag-DN-Cul1 vectors for 36 h, cells were harvested and analyzed by western for the indicated proteins. c Evi5 KO TU212 cells were transfected with control shRNA or shRNA against FBXW7 for 36 h, cells were harvested and analyzed by western for the indicated proteins. d TU212 cells were transfected with indicated plasmids for 36 h. Cells were harvested and lysates were immunoprecipitated with anti-HA antibody, and analyzed by western blot as indicated