Fig. 3

Histological analysis and growth curves of xenograft tumors derived from surgical specimens. a, b HE staining of original tumor tissue of the patient (Case 3). Original magnification: ×100 (a) and ×400 (b). c–f HE staining of the first- (c), second- (d), and third- (e) generation xenograft tissues derived from surgically resected samples. The fourth-generation xenograft is represented by the third-generation tumor that was frozen and re-implanted (f). Original magnification: ×400. g Growth of xenograft tumors, which started to increase in size at around 30 days after transplantation