Fig. 2

FTX sponged miR-335-5p in LUAD. a The location of FTX in LUAD cells was showed by FISH assay. b RNA pull-down assay examined the interaction between FTX and predicted miRNAs. c Binding sites between FTX and miR-335-5p. d MiR-335-5p expression was detected by RT-qPCR in LUAD cell lines and BEAS-2B cell line. e MiR-335-5p expression was detected in sh-FTX transfected LUAD cells. f MiR-335-5p up-regulation efficiency was examined in H1650 and H1975 cells. g Effect of miR-335-5p mimics on FTX expression was tested. h Luciferase activity of FTX-WT/Mut reporters was measured in LUAD cells transfected with miR-335-5p mimics or NC mimics. **p < 0.01, N.S. showed no significance