Table 1 The sequences for the PCR primers of genes
PCR primers | ||
|---|---|---|
Gene name | Forward primers | Reverse primers |
 FTX | GCCCATAGTGCTTCACTGGT | CATCCTTGCCTCAGAGGGA |
 miR-335-5p | TCAAGAGCAATAACGAAAAATGTG | CTCTACAGCTATATTGCCAGCCAC |
 NUCB2 | GAACGTGTTACGAGTCAGTTTTTAGT | GACAACTGAGATCCAGAGAGGTAAG |
 U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
 GAPDH | TCCCATCACCATCTTCCA | CATCACGCCACAGTTTTCC |
shRNA sequence | |
|---|---|
 sh-NC | CCGGCAGACATGATATCACATAGCTCTCGAGAGCTATGTGATATCATGTCTGTTTTTG |
 sh-FTX#1 | CCGGCACTACATCTGGCTTACACTACTCGAGTAGTGTAAGCCAGATGTAGTGTTTTTG |
 sh-FTX#2 | CCGGGACGTATACAGAATACTTCGTCTCGAGACGAAGTATTCTGTATACGTCTTTTTG |
 sh-NC | CCGGGAGTCAGTGTATAGTGCATAGCTCGAGCTATGCACTATACACTGACTCTTTTTG |
 sh-NUCB2 | CCGGAACGTGTTACGAGTCAGTTTTCTCGAGAAAACTGACTCGTAACACGTTTTTTTG |