Fig. 3

VEGF-A is a novel target gene of miR-4316 and miR-4316 inhibits VEGF-A gene expression via targeting VEGF-A 3′-UTR. a Luciferase reporter assays demonstrated that miR-4316-mimic reduced WT-VEGF-A activity in SGC-7901 and BGC-823 cells compared to MUT-VEGF-A. b qRT-PCR used to detect the expression of VEGF-A in 12 gastric cancer tissues and their adjacent normal tissues and Relative expression of VEGF-A in SGC-7901 and BGC-823 gastric cancer cell lines was determined by qRT-PCR compared with normal cells GES-1. c, d The expression of VEGF-A in GC cell lines SGC-7901 and BGC-823 cells was determined by qRT-PCR after transfection with miR-4316-mimic, inhibitor, or N.C. e The protein level of VEGF-A and c-Met were detected after 48 h transfection by miR-4316-mimic, miR-4316-inhibitor, or N.C in SGC-7901 and BGC-823 cells by western blot. f After transfection with miR-4316-mimic, miR-4316-inhibitor, or N.C in SGC-7901 and BGC-823 cells for 48 h, the fluorescence signal of VEGF-A was observed by fluorescence microscopy. Merged images were overlapped for nuclear staining by DAPI was blue color and for VEGF-A was red color. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.001