Fig. 3
From: TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin
TRIP6 enhances the stemness of breast cancer cells. a GSEA indicated significant correlations between TRIP6 mRNA expression and stemness-related gene signatures (WONG_EMBRYONIC_STEM_CELL, BHATTACHARYA_ EMBRYONIC_ STEM_CELL). b Real-time PCR and Western blot analysis of stemness-related markers in the indicated cells. Error bars represent the mean ± SD of three independent experiments; *P < 0.05. c Representative micrographs (left) and quantification (right) of mammosphere formation by TRIP6-transduced cells, TRIP6-silenced cells and vector cells. Error bars represent the mean ± SD of three independent experiments; *P < 0.05. d Real-time PCR of the mRNA expression of cancer stem cell markers. Error bars indicate the mean ± SD of three independent experiments; *P < 0.05. e TRIP6 expression levels significantly correlated with CD44 expression in breast cancer tissues (n = 340; P < 0.01). Two representative cases are shown (left) and percentage of specimens with low or high TRIP6 expression, relative to the levels of CD44 staining (right)