Fig. 6

SOS1 silencing reversed USP22-induced changes in gastric cancer cell behavior and RAS signaling. USP22-overexpressing SGC7901 cells were transfected with shSOS1. a Cell proliferation, apoptosis, migration, and invasion were evaluated. b, c Western blot analysis was performed to measure protein levels of USP22, SOS1, Ras, ERK, p-ERK, c-myc, PI3K, AKT, p-AKT, BAD, and bcl-2 in SGC7901 cells and tumor tissue. GAPDH was used as an internal control. *P < 0.05 vs. USP22-overexpressing SGC7901 cells; n = 3