Fig. 2

The effects of DANCR overexpression on cervical cancer growth. a The expression of DANCR in DANCR stably overexpressed (OV) and control HeLa cells was measured by qRT-PCR. b Cell proliferation of OV and control HeLa cells was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. c Cell proliferation of OV and control HeLa cells was analyzed by EdU assays. Red colour represents EdU-positive and proliferative cells. Scale bars = 100 μm. d After transient transfection of DANCR expressing plasmid or control empty plasmid into SiHa cells, the expression of DANCR in the transfected cells was measured by qRT-PCR. e After transient transfection of DANCR expressing plasmid or control empty plasmid into SiHa cells, cell proliferation of the transfected cells was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. f After transient transfection of DANCR expressing plasmid or control empty plasmid into SiHa cells, cell proliferation of the transfected cells was analyzed by EdU assays. Red colour represents EdU-positive and proliferative cells. Scale bars = 100 μm. For a–f, results are displayed as mean ± SD of n = 3 independent experiments. **p < 0.01 compared with Control group by Student’s t-test. g OV and control HeLa cells were subcutaneously injected into nude mice. Tumor volumes were measured every 3 days. h Subcutaneous tumor weights were detected at the twenty-first day after injection. i Proliferation marker Ki67 immunohistochemical staining in subcutaneous tumors formed by OV and control HeLa cells. Scale bars = 50 μm. For g–i, results are displayed as mean ± SD of n = 5 mice in each group. **p < 0.01 compared with Control group by Mann–Whitney test