Fig. 3

The effects of DANCR knockdown on cervical cancer growth. a The expression of DANCR in DANCR stably depleted (deleted) and control C-33A cells was measured by qRT-PCR. b Cell proliferation of deleted and control C-33A cells was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. c Cell proliferation of deleted and control C-33A cells was analyzed by EdU assays. Red colour represents EdU-positive and proliferative cells. Scale bars = 100 μm. d After transient transfection of DANCR specific shRNA (KD-DANCR) or control scrambled shRNA (KD-control) into ME-180 cells, the expression of DANCR was measured by qRT-PCR. e After transient transfection, cell proliferation of KD-DANCR and KD-control ME-180 cells was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. f After transient transfection of DANCR specific shRNA (KD-DANCR) or control scrambled shRNA (KD-control) into ME-180 cells, cell proliferation of the transfected cells was analyzed by EdU assays. Red colour represents EdU-positive and proliferative cells. Scale bars = 100 μm. For a–f, results are displayed as mean ± SD of n = 3 independent experiments. **p < 0.01 compared with KD-control group by Student’s t-test. g Deleted and control C-33A cells were subcutaneously injected into nude mice. Tumor volumes were measured every 3 days. h Subcutaneous tumor weights were detected at the twenty-first day after injection. i Proliferation marker Ki67 immunohistochemical staining in subcutaneous tumors formed by deleted and control C-33A cells. Scale bars = 50 μm. For g–i, results are displayed as mean ± SD of n = 5 mice in each group. **p < 0.01 compared with KD-control group by Mann–Whitney test