Fig. 5

The effects of DANCR on the Wnt/β-catenin signaling pathway. a Nuclear β-catenin expression levels in DANCR stably overexpressed and control HeLa cells was measured by western blot. Results are displayed as representative image of n = 3 independent experiments. b Nuclear β-catenin expression levels in DANCR stably depleted and control C-33A cells was measured by western blot. Results are displayed as representative image of n = 3 independent experiments. c The mRNA and protein expression levels of C-myc and Cyclin D1 in DANCR stably overexpressed and control HeLa cells were measured by qRT-PCR and western blot. Results are displayed as mean ± SD of n = 3 independent experiments. **p < 0.01 compared with Control group by Student’s t-test. d The mRNA and protein expression levels of C-myc and Cyclin D1 in DANCR stably depleted and control C-33A cells were measured by qRT-PCR and western blot. Results are displayed as mean ± SD of n = 3 independent experiments. **p < 0.01, ***p < 0.001 compared with KD-control group by Student’s t-test