Fig. 6

Wnt pathway inhibitor ICG-001 abolishes the roles of DANCR in cervical cancer growth. a DANCR stably overexpressed and control HeLa cells were treated with 5 μM ICG-001. Concurrently, cell proliferation was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. b DANCR stably overexpressed and control HeLa cells were treated with 5 μM ICG-001. Concurrently, cell proliferation was analyzed by EdU assays. Red color represents EdU-positive and proliferative cells. Scale bars = 100 μm. For a, b, results are displayed as mean ± SD of n = 3 independent experiments. ns, not significant, compared with Control + ICG-001 group by Student’s t-test. c DANCR stably depleted and control C-33A cells were treated with 5 μM ICG-001. Concurrently, cell proliferation was analyzed by CCK-8 assays. OD 450 values at indicated time points were displayed to indicate cell proliferation. d DANCR stably depleted and control C-33A cells were treated with 5 μM ICG-001. Concurrently, cell proliferation was analyzed by EdU assays. Red color represents EdU-positive and proliferative cells. Scale bars = 100 μm. For c, d, results are displayed as mean ± SD of n = 3 independent experiments. ns, not significant, compared with KD-control + ICG-001 group by Student’s t-test