Fig. 2

KMT9 controls expression of genes involved in the organization of organelles, cells death and cell proliferation. a Venn diagram showing overlap and number of genes/proteins in A549 cells that are differentially expressed upon RNAi mediated knock-down of KMT9α (log2(fold-change) > ± 0.26). In total, 460 targets are concomitantly up- or down-regulated on RNA and protein level upon knock-down of KMT9α. Enriched GO_cellular components b and GO_biological processes c gene sets obtained for the indicated 460 KMT9α-regulated target genes. d Heat map displaying mRNA levels of the 460 KMT9α-regulated genes involved in cell proliferation (GO:0042127) in A549 cells treated with siControl or siKMT9α#1. e RNA sequencing reads (left panel) and mass spectrometry volcano plot (right panel) for the indicated genes and proteins are represented exemplarily. f Western blot displaying expression of the target proteins indicated in e upon knock-down of KMT9α in A549 cells. The indicated antibodies were used. g Quantitative real-time PCR analysis of the mRNA expression of the target genes displayed in e after knock-down of KMT9α. Data represent means + standard deviation. Significance was accessed by two-tailed t test, n = 3 (TIMP2 n = 6)