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Fig. 3 | Cancer Cell International

Fig. 3

From: Depletion of histone methyltransferase KMT9 inhibits lung cancer cell proliferation by inducing non-apoptotic cell death

Fig. 3

Knock-down of KMT9α inhibits A549 lung cancer cell proliferation and induces non-apoptotic cell death. a, b The granularity of A549 cells with siControl or siKMT9α#1 was measured by flow cytometry using side scatter (SSC). a Figure exemplifying the gating strategy used to assess size of “high granularity” population of A549 cells treated with siControl and siKMT9α#1. b Column graph showing the percentage of A549 cells with “high granularity” upon treatment with siControl or siKMT9α#1. Data represent means + standard deviation. Significance was accessed by two-tailed paired t test, n = 3. ce Proliferation assay. c Representative proliferation curve of A549 cells treated with siControl or siKMT9α#1. Data represent mean ± standard deviation. The experiment was repeated three times independently. d Column graph representing the population doubling time (0–72 h). Data represent means + standard deviation. Significance was accessed by two-tailed paired t test, n = 3. e Column graph representing the percentage of living cells 72 h after starting the experiment. Data represent means + standard deviation. Significance was accessed by two-tailed paired t test, n = 3. f, g Apoptosis assay. Apoptotic A549 cells treated with siControl or siKMT9α#1 were identified by flow cytometry using Annexin V and 7-AAD staining. f Day 2 of one representative experiment is shown. g Bar graphs representing the percentage of A549 apoptotic cells upon treatment with siControl or siKMT9α#1 on days 1–3. Columns represent means + standard deviation. Significance was assessed by two-tailed paired t test, n = 3. h, i Cell cycle phase distribution was assessed in A549 cells treated with siControl or siKMT9α#1 by propidium iodide (PI) staining and flow cytometry. h Day 3 of one representative experiment is shown. i Bar graphs representing percentage of cells per cell cycle phase in A549 cells treated with siControl or siKMT9α#1 on days 1–3. Data represent means + standard deviation. Significance was assessed by two-tailed paired t test, n = 3. SSC side scatter, FSC forward scatter; ns: not significant

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