Fig. 2

Overexpression of FER1L4 inhibited cell proliferation and induced cell apoptosis in prostate cancer cells. a, b RT-qPCR was performed to detect the transfection efficiency of pcDNA3-FER1L4 in PC-3 and DU145 cells. Transfection of pcDNA3-FER1L4 elevated FER1L4 expression in PC-3 (a) and DU145 (b) cells. c, d The CCK-8 assay was used to detect the cell proliferation ability. Overexpression of FER1L4 decreased absorbance at OD 450 nM, indicating inhibition of cell proliferation in PC-3 (c) and DU145 (d) cells. e, f Flow cytometry was used to analyze the percentage of apoptotic cells. Overexpression of FER1L4 increased percentage of cells positive for PI staining, indicating induction of apoptosis in PC-3 (e) and DU145 (f) cells. g, h Overexpression of FER1L4 increased caspase3 activity in PC-3 (g) and DU145 cells (h). i Western blotting showed that overexpression of FER1L4 decreased Bcl-2 and increased Bax protein expression in PC-3 and DU145 cells. ***p < 0.001