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Fig. 1 | Cancer Cell International

Fig. 1

From: MEX3A knockdown inhibits the development of pancreatic ductal adenocarcinoma

Fig. 1

MEX3A is highly expressed in PDA and the construction of MEX3A knockdown cell model. a Expression levels of MEX3A in PDA tumor tissues and adjacent normal skin tissues were detected by IHC staining. b Kaplan–Meier survival analysis MEX3A expression and overall survival of PDA. c MEX3A expression in HPDE6-C7, BxPc-3, PANC-1 and SW1990 cells was detected by qRT-PCR. d qRT-PCR was used to screen knockdown efficiency of MEX3A in shMEX3A-1, shMEX3A-2, and shMEX3A-3 groups. e Infection efficiency for PANC-1 and SW1990 cells was evaluated by expression of green fluorescent protein 72 h post-infection. f, g The specificity and validity of the lentivirus-mediated shRNA knockdown of MEX3A expression was verified by qRT-PCR (f) and western blot analysis (g). The data were presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001

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