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Fig. 2 | Cancer Cell International

Fig. 2

From: Circ_0032821 acts as an oncogene in cell proliferation, metastasis and autophagy in human gastric cancer cells in vitro and in vivo through activating MEK1/ERK1/2 signaling pathway

Fig. 2

Knockdown of circ_0032821 suppressed cell proliferation, epithelial-mesenchymal transition (EMT), migration and invasion in human GC cells in vitro. AGS and HGC-27 cells were transfected with siRNA against circ_0032821 (si-circ_0032821) or its scrambled sequence (si-NC). a RT-qPCR detected circ_0032821 level after transfection for 1 day. b, c Cell proliferative ability was assessed by Cell counting kit (CCK)-8 after transfection for 3 days. Optimal density (OD) values at 450 nm were recorded at 0 day, 1 day, 2 days, and 3 days. d, e Cell migratory and invasive abilities were evaluated by transwell assays. The migrated cells and invaded cells were counted after transfection for 1 day. One representative image (×200) in each group was presented. f, g Western blotting measured protein expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase 2 (MMP2) and MMP9 in transfected AGS and HGC-27 cells on 1 day. h, i Western blotting measured protein expression of E-cadherin, N-cadherin and Vimentin in transfected AGS and HGC-27 cells on 1 day. Relative protein expression was normalized to β-actin. *P < 0.05

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