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Fig. 2 | Cancer Cell International

Fig. 2

From: Sirtuin 1 inhibits lipopolysaccharide-induced inflammation in chronic myelogenous leukemia k562 cells through interacting with the Toll-like receptor 4-nuclear factor κ B-reactive oxygen species signaling axis

Fig. 2

SIRT1 expression in LPS-treated k562 cells. a qPCR was used to measure the mRNA expression of SIRT1 at 0, 4, 8, and 12 h after LPS treatment in k562 cells. b, c WB was performed to detect SIRT1 protein levels in k562 and KU812 cells after LPS treatment. d SIRT1 subcellular localization in LPS-treated k562 cells detected using IFA. SIRT1 was stained to red using anti-SIRT1 antibody (red), whereas nuclear DNA was counterstained blue using DAPI (blue). The merged image displays the nuclear localization of SIRT1. e Cell fractionation assay showed the location of SIRT1 in nuclear and cytoplasmic fractions. f SIRT1 activity was detected in the whole cell lysate. Data are expressed as mean ± standard deviation. *P < 0.05, **P < 0.01 relative to the control

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Cancer Cell International

ISSN: 1475-2867

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